Effect of ?-Estradiol on Mono- and Mixed-Species Biofilms of Human Commensal Bacteria Lactobacillus paracasei AK508 and Micrococcus luteus C01 on Different Model Surfaces

The impact of steroid hormones, and particularly estradiol, on human microbiota could be recognized as a substantial part human-microbiota interactions. However, an area that remains poorly investigated is the skin vaginal microbial communities biofilms, which contain non-pathogenic bacteria phyla Firmicutes Actinobacteria, especially probiotic genus Lactobacillus widespread, safe genus, Micrococcus. Experiments with paracasei AK508 Micrococcus luteus C01 biofilms PTFE cubes showed dose-dependent effects estradiol at concentrations 0.22 nM 22 nM. hormone mostly inhibits L. growth stimulates M. luteus. presented studies colony-forming unit (CFU) amountsand cell aggregation in glass fiber filters same general tendencies. Estradiol generally increased cells monospecies potentially changed synthesis antibacterial metabolites paracasei. balance between two mixed-species depended initial adhesion stage, when this stage was reduced, micrococci were more resistant to antagonistic action Moreover, effect lactobacilli altered from inhibition stimulation, due presence At time, ethanol solvent for concentration 0.6% acted antagonist had opposite bacteria; nevertheless, overlapping shown minimal. data obtained prove complexity interactions regulatory commensal biofilms.